› 6zoe

X-ray diffraction
2.85Å resolution

AcrB-F563A symmetric T protomer

Released:
DOI: 10.2210/pdb6zoe/pdb
PDB entry 6zoe coloured by chain, front view.
PDB entry 6zoe coloured by chain, side view.
PDB entry 6zoe coloured by chain, top view.
Source organisms:
Primary publication:
Allosteric drug transport mechanism of multidrug transporter AcrB.
Tam HK, Foong WE, Oswald C, Herrmann A, Zeng H, Pos KM
Nat Commun 12 3889 (2021)
PMID: 34188038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151962 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Multidrug efflux pump subunit AcrB Chain: A
Molecule details ›
Chain: A
Length: 1057 amino acids
Theoretical weight: 114.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31224 (Residues: 1-1049; Coverage: 100%)
Gene names: JW0451, acrB, acrE, b0462
Sequence domains: AcrB/AcrD/AcrF family
DARPIN Chain: B
Molecule details ›
Chain: B
Length: 169 amino acids
Theoretical weight: 18.32 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

4 bound ligands:
Ligand D10 1 x D10
Ligand EDO 2 x EDO
Ligand LMT 1 x LMT
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I23
Unit cell:
a: 226.609Å b: 226.609Å c: 226.609Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.265
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12

Quick links

Citations

8 review citations

Molecular mechanisms of antibiotic resistance revisited.
Darby et al. (2023)
7 more