› 6zni

Electron Microscopy
3.6Å resolution

Structure of the Shigella MxiH needle filament attached to the basal body

Released:
DOI: 10.2210/pdb6zni/pdb
PDB entry 6zni coloured by chain, front view.
PDB entry 6zni coloured by chain, side view.
PDB entry 6zni coloured by chain, top view.
Source organism: Shigella flexneri 5a str. M90T
Primary publication:
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.
Kotov V, Lunelli M, Wald J, Kolbe M, Marlovits TC
OpenAccess logo Biochem Biophys Rep 27 101039 (2021)
PMID: 34258394
Related structures: EMD-11312

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 23-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type 3 secretion system needle filament protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W
Length: 98 amino acids
Theoretical weight: 11.06 KDa
Source organism: Shigella flexneri 5a str. M90T
Expression system: Shigella flexneri 5a str. M90T
UniProt:
  • Canonical: P0A223 (Residues: 1-83; Coverage: 100%)
Gene names: CP0137, mxiH, sctF
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-11312
Expression system: Shigella flexneri 5a str. M90T

Quick links

Citations

2 review citations

Cryo-EM of the injectisome and type III secretion systems.
Bergeron et al. (2022)
1 more

1 mention without citation

Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.
Kotov et al. (2021)