› 6zir

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF NRAS (C118S) IN COMPLEX WITH GDP AND COMPOUND 18

Released:
DOI: 10.2210/pdb6zir/pdb
PDB entry 6zir coloured by chain, front view.
PDB entry 6zir coloured by chain, side view.
PDB entry 6zir coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Drugging all RAS isoforms with one pocket.
Kessler D, Bergner A, Böttcher J, Fischer G, Döbel S, Hinkel M, Müllauer B, Weiss-Puxbaum A, McConnell DB
Future Med Chem 12 1911-1923 (2020)
PMID: 32779487

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133953 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase NRas Chain: A
Molecule details ›
Chain: A
Length: 173 amino acids
Theoretical weight: 19.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01111 (Residues: 1-172; Coverage: 91%)
Gene names: HRAS1, NRAS
Sequence domains: Ras family

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GDP 1 x GDP
Ligand EZZ 2 x EZZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P321
Unit cell:
a: 103.806Å b: 103.806Å c: 54.775Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.215
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The Ins and Outs of RAS Effector Complexes.
Kiel et al. (2021)
7 more