› 6zi7

X-ray diffraction
2.28Å resolution

Crystal structure of OleP-oleandolide(DEO) bound to L-rhamnose

Released:
DOI: 10.2210/pdb6zi7/pdb
PDB 6zi7 coloured by chain and viewed from the front.
PDB 6zi7 coloured by chain and viewed from the side.
PDB 6zi7 coloured by chain and viewed from the top.
Source organism: Streptomyces antibioticus
Primary publication:
Dissecting the Cytochrome P450 OleP Substrate Specificity: Evidence for a Preferential Substrate.
Parisi G, Freda I, Exertier C, Cecchetti C, Gugole E, Cerutti G, D'Auria L, Macone A, Vallone B, Savino C, Montemiglio LC
Biomolecules 10 (2020)
PMID: 33036250

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P-450 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 407 amino acids
Theoretical weight: 45.01 KDa
Source organism: Streptomyces antibioticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q59819 (Residues: 1-407; Coverage: 100%)
Gene name: oleP
Sequence domains: Cytochrome P450

Ligands and Environments

5 bound ligands:
Ligand HEM 6 x HEM
Ligand QR8 6 x QR8
Ligand RAM 3 x RAM
Ligand FMT 214 x FMT
Ligand NA 6 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: C2
Unit cell:
a: 247.531Å b: 110.683Å c: 159.285Å
α: 90° β: 129.46° γ: 90°
R-values:
R R work R free
0.187 0.184 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Point Mutations at a Key Site Alter the Cytochrome P450 OleP Structural Dynamics.
Montemiglio et al. (2021)