6zhs

X-ray diffraction
2.35Å resolution

Uba1 bound to two E2 (Ubc13) molecules

Released:
Entry authors: Misra M, Schindelin H

Function and Biology Details

Reactions catalysed:
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-activating enzyme E1 1 Chain: A
Molecule details ›
Chain: A
Length: 1024 amino acids
Theoretical weight: 114.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P22515 (Residues: 1-1024; Coverage: 100%)
Gene names: UBA1, YKL210W
Sequence domains:
Ubiquitin-conjugating enzyme E2 13 Chains: B, C
Molecule details ›
Chains: B, C
Length: 154 amino acids
Theoretical weight: 17.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P52490 (Residues: 1-153; Coverage: 100%)
Gene names: UBC13, YD6652.04, YDR092W
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 177.71Å b: 72.9Å c: 139.52Å
α: 90° β: 112.43° γ: 90°
R-values:
R R work R free
0.219 0.216 0.275
Expression system: Escherichia coli