6zhe

Electron Microscopy
7.24Å resolution

Cryo-EM structure of DNA-PK dimer

Released:
Source organism: Homo sapiens
Related structures: EMD-11219

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-121014 (preferred)
Entry contents:
3 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (7 distinct):
DNA-dependent protein kinase catalytic subunit Chains: A, F
Molecule details ›
Chains: A, F
Length: 4156 amino acids
Theoretical weight: 472.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P78527 (Residues: 1-4128; Coverage: 100%)
Gene names: HYRC, HYRC1, PRKDC
Sequence domains:
X-ray repair cross-complementing protein 6 Chains: B, G
Molecule details ›
Chains: B, G
Length: 609 amino acids
Theoretical weight: 69.95 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P12956 (Residues: 1-609; Coverage: 100%)
Gene names: G22P1, XRCC6
Sequence domains:
X-ray repair cross-complementing protein 5 Chains: C, H
Molecule details ›
Chains: C, H
Length: 732 amino acids
Theoretical weight: 82.81 KDa
Source organism: Homo sapiens
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: P13010 (Residues: 1-732; Coverage: 100%)
Gene names: G22P2, XRCC5
Sequence domains:
DNA (25-MER) Chain: J
Molecule details ›
Chain: J
Length: 25 nucleotides
Theoretical weight: 7.75 KDa
DNA (27-MER) Chain: I
Molecule details ›
Chain: I
Length: 27 nucleotides
Theoretical weight: 8.29 KDa
DNA (26-MER) Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
DNA (28-MER) Chain: E
Molecule details ›
Chain: E
Length: 28 nucleotides
Theoretical weight: 8.6 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.24Å
Relevant EMDB volumes: EMD-11219
Expression system: Insect cell expression vector pTIE1