6zg3

X-ray diffraction
2.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Energy-coupling factor transporter ATP-binding protein EcfA1 Chains: A, F
Molecule details ›
Chains: A, F
Length: 300 amino acids
Theoretical weight: 33.17 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GBJ0 (Residues: 2-282; Coverage: 100%)
Gene names: Ldb0424, cbiO1, ecfA1
Sequence domains: ABC transporter
Energy-coupling factor transporter ATP-binding protein EcfA2 Chains: B, G
Molecule details ›
Chains: B, G
Length: 287 amino acids
Theoretical weight: 31.67 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GBI9 (Residues: 1-287; Coverage: 100%)
Gene names: Ldb0425, cbiO2, ecfA2
Sequence domains: ABC transporter
Conserved hypothetical membrane protein Chains: C, H
Molecule details ›
Chains: C, H
Length: 215 amino acids
Theoretical weight: 23.01 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GBG0 (Residues: 1-207; Coverage: 100%)
Gene name: Ldb0463
Sequence domains: ECF transporter, substrate-specific component
Energy-coupling factor transporter transmembrane protein EcfT Chains: D, I
Molecule details ›
Chains: D, I
Length: 265 amino acids
Theoretical weight: 30.29 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 = JCM 1002
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GBI8 (Residues: 1-265; Coverage: 100%)
Gene names: Ldb0426, cbiQ, ecfT
Sequence domains: Cobalt transport protein
CA14381 nanobody Chains: E, J
Molecule details ›
Chains: E, J
Length: 136 amino acids
Theoretical weight: 14.98 KDa
Source organism: Lama glama

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 97.29Å b: 110.47Å c: 110.5Å
α: 88.997° β: 102.271° γ: 102.244°
R-values:
R R work R free
0.244 0.242 0.276
Expression system: Escherichia coli