› 6zfc

X-ray diffraction
1.65Å resolution

Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative

Released:
DOI: 10.2210/pdb6zfc/pdb
PDB entry 6zfc coloured by chain, front view.
PDB entry 6zfc coloured by chain, side view.
PDB entry 6zfc coloured by chain, top view.
Source organism: Burkholderia ambifaria AMMD
Primary publication:
Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for Burkholderia ambifaria lectin (BambL).
Kuhaudomlarp S, Cerofolini L, Santarsia S, Gillon E, Fallarini S, Lombardi G, Denis M, Giuntini S, Valori C, Fragai M, Imberty A, Dondoni A, Nativi C
Chem Sci 11 12662-12670 (2020)
PMID: 34094460

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-binding lectin protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 87 amino acids
Theoretical weight: 9.39 KDa
Source organism: Burkholderia ambifaria AMMD
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0B4G1 (Residues: 1-87; Coverage: 100%)
Gene name: Bamb_5415
Sequence domains: Fungal fucose-specific lectin

Ligands and Environments

1 bound ligand:
Ligand QJB 12 x QJB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Unit cell:
a: 109.092Å b: 49.163Å c: 115.098Å
α: 90° β: 91.36° γ: 90°
R-values:
R R work R free
0.178 0.177 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Druggable Allosteric Sites in β-Propeller Lectins.
Shanina et al. (2022)
1 more

2 mentions without citation

Glycomimetics for the inhibition and modulation of lectins.
Leusmann et al. (2023)
1 more