› 6z3o

X-ray diffraction
1.74Å resolution

The E74Q mutant of Small Alarmone Hydrolase SAH from Pseudomonas aeruginosa PAO1

Released:
DOI: 10.2210/pdb6z3o/pdb
PDB entry 6z3o coloured by chain, front view.
PDB entry 6z3o coloured by chain, side view.
PDB entry 6z3o coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Jin Y, Rizkallah P, Bell H

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional (P)ppGpp synthetase/guanosine-3',5'-bis(Diphosphate) 3'-pyrophosphohydrolase Chains: AAA, BBB, CCC, DDD
Molecule details ›
Chains: AAA, BBB, CCC, DDD
Length: 205 amino acids
Theoretical weight: 23.11 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I686 (Residues: 1-184; Coverage: 100%)
Gene name: PA0431
Sequence domains: HD domain

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 86.15Å b: 86.05Å c: 122.58Å
α: 90° β: 101.77° γ: 90°
R-values:
R R work R free
0.156 0.152 0.227
Expression system: Escherichia coli BL21(DE3)

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