› 6z10

X-ray diffraction
2.27Å resolution

Crystal structure of a humanized (K18E, K269N) rat succinate receptor SUCNR1 (GPR91) in complex with a nanobody and antagonist

Released:
DOI: 10.2210/pdb6z10/pdb
PDB 6z10 coloured by chain and viewed from the front.
PDB 6z10 coloured by chain and viewed from the side.
PDB 6z10 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Discovery and Optimization of Novel SUCNR1 Inhibitors: Design of Zwitterionic Derivatives with a Salt Bridge for the Improvement of Oral Exposure.
Velcicky J, Wilcken R, Cotesta S, Janser P, Schlapbach A, Wagner T, Piechon P, Villard F, Bouhelal R, Piller F, Harlfinger S, Stringer R, Fehlmann D, Kaupmann K, Littlewood-Evans A, Haffke M, Gommermann N
J Med Chem 63 9856-9875 (2020)
PMID: 32856916

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 39.83 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6IYF9 (Residues: 2-317; Coverage: 100%)
Gene names: Gpr91, Sucnr1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody6 Chain: B
Molecule details ›
Chain: B
Length: 142 amino acids
Theoretical weight: 15.53 KDa
Source organism: Vicugna pacos
Expression system: Spodoptera frugiperda

Ligands and Environments

4 bound ligands:
Ligand Q4T 1 x Q4T
Ligand OLC 12 x OLC
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 76.839Å b: 151.003Å c: 68.211Å
α: 90° β: 112.29° γ: 90°
R-values:
R R work R free
0.205 0.204 0.223
Expression system: Spodoptera frugiperda

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