X-ray diffraction
1.31Å resolution

Crystal structure of SAICAR Synthetase (PurC) from Mycobacterium abscessus in complex with fragment 1


Function and Biology Details

Reaction catalysed:
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphoribosylaminoimidazole-succinocarboxamide synthase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.9 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
  • Canonical: B1MHW4 (Residues: 1-297; Coverage: 100%)
Gene names: MAB_0689, purC
Sequence domains: SAICAR synthetase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 47.493Å b: 64.729Å c: 48.238Å
α: 90° β: 110.88° γ: 90°
R R work R free
0.181 0.18 0.202
Expression system: Escherichia coli BL21(DE3)