› 6yx6

X-ray diffraction
1.5Å resolution

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (no morpholine)

Released:
DOI: 10.2210/pdb6yx6/pdb
PDB entry 6yx6 coloured by chain, front view.
PDB entry 6yx6 coloured by chain, side view.
PDB entry 6yx6 coloured by chain, top view.
Source organism: Chloroflexus aggregans DSM 9485
Primary publication:
The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.
Röllen K, Granzin J, Remeeva A, Davari MD, Gensch T, Nazarenko VV, Kovalev K, Bogorodskiy A, Borshchevskiy V, Hemmer S, Schwaneberg U, Gordeliy V, Jaeger KE, Batra-Safferling R, Gushchin I, Krauss U
J Biol Chem 296 100662
PMID: 33862085

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.4 KDa
Source organism: Chloroflexus aggregans DSM 9485
Expression system: Escherichia coli
UniProt:
  • Canonical: B8GAY9 (Residues: 47-153; Coverage: 12%)
Gene name: Cagg_3753
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 57.772Å b: 110.839Å c: 39.197Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Light-regulated gene expression in Bacteria: Fundamentals, advances, and perspectives.
Ohlendorf et al. (2022)
5 other articles

1 mention without citation

The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins.
Röllen et al. (2021)