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Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.

Itoh Y, Naschberger A, Mortezaei N, Herrmann JM, Amunts A
Nat Commun 11 5187 (2020)
Related entries: 6yw5, 6ywe, 6yws, 6ywv, 6ywy

Cited: 21 times
EuropePMC logo PMID: 33056988

Abstract

Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF1) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.

Articles - 6ywx mentioned but not cited (1)

  1. Structure of a mitochondrial ribosome with fragmented rRNA in complex with membrane-targeting elements. Tobiasson V, Berzina I, Amunts A. Nat Commun 13 6132 (2022)


Reviews citing this publication (3)

  1. The Diseased Mitoribosome. Ferrari A, Del'Olio S, Barrientos A. FEBS Lett 595 1025-1061 (2021)
  2. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)
  3. Types and Functions of Mitoribosome-Specific Ribosomal Proteins across Eukaryotes. Scaltsoyiannes V, Corre N, Waltz F, Giegé P. Int J Mol Sci 23 3474 (2022)

Articles citing this publication (17)

  1. Mechanism of membrane-tethered mitochondrial protein synthesis. Itoh Y, Andréll J, Choi A, Richter U, Maiti P, Best RB, Barrientos A, Battersby BJ, Amunts A. Science 371 846-849 (2021)
  2. Structural basis of mitochondrial translation. Aibara S, Singh V, Modelska A, Amunts A. Elife 9 e58362 (2020)
  3. Interconnected assembly factors regulate the biogenesis of mitoribosomal large subunit. Tobiasson V, Gahura O, Aibara S, Baradaran R, Zíková A, Amunts A. EMBO J 40 e106292 (2021)
  4. Mechanism of mitoribosomal small subunit biogenesis and preinitiation. Itoh Y, Khawaja A, Laptev I, Cipullo M, Atanassov I, Sergiev P, Rorbach J, Amunts A. Nature 606 603-608 (2022)
  5. An Unexpectedly Complex Mitoribosome in Andalucia godoyi, a Protist with the Most Bacteria-like Mitochondrial Genome. Valach M, Gonzalez Alcazar JA, Sarrasin M, Lang BF, Gray MW, Burger G. Mol Biol Evol 38 788-804 (2021)
  6. How to build a ribosome from RNA fragments in Chlamydomonas mitochondria. Waltz F, Salinas-Giegé T, Englmeier R, Meichel H, Soufari H, Kuhn L, Pfeffer S, Förster F, Engel BD, Giegé P, Drouard L, Hashem Y. Nat Commun 12 7176 (2021)
  7. Functional analyses of mitoribosome 54S subunit devoid of mitochondria-specific protein sequences. Santos B, Zeng R, Jorge SF, Ferreira-Junior JR, Barrientos A, Barros MH. Yeast 39 208-229 (2022)
  8. Lone Pair…π Contacts and Structure Signatures of r(UNCG) Tetraloops, Z-Turns, and Z-Steps: A WebFR3D Survey. Zirbel CL, Auffinger P. Molecules 27 4365 (2022)
  9. Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway. Valach M, Benz C, Aguilar LC, Gahura O, Faktorová D, Zíková A, Oeffinger M, Burger G, Gray MW, Lukeš J. Nucleic Acids Res 51 6443-6460 (2023)
  10. Principles of mitoribosomal small subunit assembly in eukaryotes. Harper NJ, Burnside C, Klinge S. Nature 614 175-181 (2023)
  11. Structural analysis of mitochondrial rRNA gene variants identified in patients with deafness. Vila-Sanjurjo A, Mallo N, Elson JL, Smith PM, Blakely EL, Taylor RW. Front Physiol 14 1163496 (2023)
  12. Structure of the mitoribosomal small subunit with streptomycin reveals Fe-S clusters and physiological molecules. Itoh Y, Singh V, Khawaja A, Naschberger A, Nguyen MD, Rorbach J, Amunts A. Elife 11 e77460 (2022)
  13. Structure of the translating Neurospora ribosome arrested by cycloheximide. Shen L, Su Z, Yang K, Wu C, Becker T, Bell-Pedersen D, Zhang J, Sachs MS. Proc Natl Acad Sci U S A 118 e2111862118 (2021)
  14. The dynamics of subunit rotation in a eukaryotic ribosome. Freitas FC, Fuchs G, de Oliveira RJ, Whitford PC. Biophysica 1 204-221 (2021)
  15. Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling. Key J, Gispert S, Koepf G, Steinhoff-Wagner J, Reichlmeir M, Auburger G. Int J Mol Sci 24 17503 (2023)
  16. Translational activators and mitoribosomal isoforms cooperate to mediate mRNA-specific translation in Schizosaccharomyces pombe mitochondria. Herbert CJ, Labarre-Mariotte S, Cornu D, Sophie C, Panozzo C, Michel T, Dujardin G, Bonnefoy N. Nucleic Acids Res 49 11145-11166 (2021)
  17. Widespread use of unconventional targeting signals in mitochondrial ribosome proteins. Bykov YS, Flohr T, Boos F, Zung N, Herrmann JM, Schuldiner M. EMBO J 41 e109519 (2022)


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