› 6ywh

X-ray diffraction
1.07Å resolution

Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148D variant

Released:
DOI: 10.2210/pdb6ywh/pdb
PDB entry 6ywh coloured by chain, front view.
PDB entry 6ywh coloured by chain, side view.
PDB entry 6ywh coloured by chain, top view.
Source organism: Chloroflexus aggregans DSM 9485
Primary publication:
Insights into the mechanisms of light-oxygen-voltage domain color tuning from a set of high-resolution X-ray structures.
Remeeva A, Nazarenko VV, Kovalev K, Goncharov IM, Yudenko A, Astashkin R, Gordeliy V, Gushchin I
Proteins (2021)
PMID: 33774867

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.39 KDa
Source organism: Chloroflexus aggregans DSM 9485
Expression system: Escherichia coli
UniProt:
  • Canonical: B8GAY9 (Residues: 47-153; Coverage: 12%)
Gene name: Cagg_3753
Sequence domains: PAS domain

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand MES 1 x MES
Ligand PG4 1 x PG4
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 53.661Å b: 110.471Å c: 39.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.188
Expression system: Escherichia coli

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Citations

1 review citation

Current Trends of Bacterial and Fungal Optoproteins for Novel Optical Applications.
Ramírez Martínez et al. (2023)
5 other articles