6ysw

X-ray diffraction
2.82Å resolution

E. coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme A

Released:
Source organism: Escherichia coli K-12
Entry authors: Sah-Teli SK, Hynonen MJ, Wierenga RK, Venkatesan R

Function and Biology Details

Reactions catalysed:
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid oxidation complex subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 728 amino acids
Theoretical weight: 78.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77399 (Residues: 1-714; Coverage: 100%)
Gene names: JW2338, b2341, fadJ, yfcX
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 204.793Å b: 90.724Å c: 128.625Å
α: 90° β: 123.165° γ: 90°
R-values:
R R work R free
0.203 0.2 0.254
Expression system: Escherichia coli