X-ray diffraction
2.7Å resolution

E. coli anaerobic trifunctional enzyme subunit-alpha

Source organism: Escherichia coli K-12
Entry authors: Sah-Teli SK, Hynonen MJ, Wierenga RK, Venkatesan R

Function and Biology Details

Reactions catalysed:
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Fatty acid oxidation complex subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 728 amino acids
Theoretical weight: 78.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P77399 (Residues: 1-714; Coverage: 100%)
Gene names: JW2338, b2341, fadJ, yfcX
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 128.583Å b: 90.751Å c: 172.912Å
α: 90° β: 96.112° γ: 90°
R R work R free
0.225 0.224 0.254
Expression system: Escherichia coli