X-ray diffraction
2.5Å resolution

SHMT from Streptococcus thermophilus Tyr55Ser variant as internal aldimine and as non-covalent complex with D-Ser

Source organism: Streptococcus thermophilus
Entry authors: Petrillo G, Hernandez K, Bujons J, Clapes P, Uson I

Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Serine hydroxymethyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 416 amino acids
Theoretical weight: 45.06 KDa
Source organism: Streptococcus thermophilus
Expression system: Escherichia coli
  • Canonical: Q5M0B4 (Residues: 1-416; Coverage: 100%)
Gene names: glyA, str0755
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 199.956Å b: 113.657Å c: 132.479Å
α: 90° β: 94.13° γ: 90°
R R work R free
0.179 0.176 0.226
Expression system: Escherichia coli