X-ray diffraction
2.42Å resolution

X-ray structure of the K72I, Y129F, R133L, H199A quadruple mutant of PNP-oxidase from E. coli in complex with PLP


Function and Biology Details

Reaction catalysed:
Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Pyridoxine/pyridoxamine 5'-phosphate oxidase Chains: AAA, BBB
Molecule details ›
Chains: AAA, BBB
Length: 218 amino acids
Theoretical weight: 25.44 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P0AFI7 (Residues: 1-218; Coverage: 100%)
Gene names: JW1630, b1638, pdxH
Sequence domains:

Ligands and Environments

Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P61
Unit cell:
a: 54.164Å b: 54.164Å c: 271.968Å
α: 90° β: 90° γ: 120°
R R work R free
0.193 0.19 0.259
Expression system: Escherichia coli