› 6ylr

X-ray diffraction
2.2Å resolution

Translation initiation factor 4E in complex with bn7GpppG mRNA 5' cap analog

Released:
DOI: 10.2210/pdb6ylr/pdb
PDB entry 6ylr coloured by chain, front view.
PDB entry 6ylr coloured by chain, side view.
PDB entry 6ylr coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Novel N7-Arylmethyl Substituted Dinucleotide mRNA 5' cap Analogs: Synthesis and Evaluation as Modulators of Translation.
Wojcik R, Baranowski MR, Markiewicz L, Kubacka D, Bednarczyk M, Baran N, Wojtczak A, Sikorski PJ, Zuberek J, Kowalska J, Jemielity J
Pharmaceutics 13 (2021)
PMID: 34834356

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 22.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63073 (Residues: 28-217; Coverage: 88%)
Gene name: Eif4e
Sequence domains: Eukaryotic initiation factor 4E

Ligands and Environments

1 bound ligand:
Ligand OYE 2 x OYE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43
Unit cell:
a: 94.047Å b: 94.047Å c: 42.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Pivotal Role of Chemical Modifications in mRNA Therapeutics.
Liu et al. (2022)
6 other articles