› 6yh0

X-ray diffraction
1.56Å resolution

Marasmius oreades agglutinin (MOA) in complex with the truncated PVPRAHS synthetic substrate

Released:
DOI: 10.2210/pdb6yh0/pdb
PDB entry 6yh0 coloured by chain, front view.
PDB entry 6yh0 coloured by chain, side view.
PDB entry 6yh0 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of MOA in complex with a peptide fragment: A protease caught in flagranti.
Manna D, Cordara G, Krengel U
Curr Res Struct Biol 2 56-67 (2020)
PMID: 34235469

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167878 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Agglutinin Chain: AAA
Molecule details ›
Chain: AAA
Length: 293 amino acids
Theoretical weight: 32.35 KDa
Source organism: Marasmius oreades
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q8X123 (Residues: 1-293; Coverage: 100%)
Sequence domains:
PRO-VAL-PRO-ARG Chain: EEE
Molecule details ›
Chain: EEE
Length: 7 amino acids
Theoretical weight: 765 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: FUC, GLA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLA
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand CZZ 1 x CZZ

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 121.244Å b: 121.244Å c: 99.913Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.199
Expression systems:
  • Escherichia coli K-12
  • Not provided

Quick links

Citations

1 citations in other articles

Elucidating arsenic-bound proteins in the protein data bank: data mining and amino acid cross-validation through Raman spectroscopy.
Nayek et al. (2023)

1 mention without citation

Crystal structure of MOA in complex with a peptide fragment: A protease caught in flagranti.
Manna et al. (2020)