X-ray diffraction
2.66Å resolution

Human Ecto-5'-nucleotidase (CD73) in complex with the AMPCP derivative A894 (compound 2n in publication) in the closed form (crystal form IV)


Function and Biology Details

Reaction catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
5'-nucleotidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 532 amino acids
Theoretical weight: 59.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P21589 (Residues: 27-549; Coverage: 95%)
Gene names: NT5, NT5E, NTE
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21212
Unit cell:
a: 93.03Å b: 232.12Å c: 53.79Å
α: 90° β: 90° γ: 90°
R R work R free
0.197 0.193 0.268
Expression system: Escherichia coli