› 6ydf

X-ray diffraction
2.12Å resolution

X-ray structure of LPMO.

Released:
DOI: 10.2210/pdb6ydf/pdb
PDB entry 6ydf coloured by chain, front view.
PDB entry 6ydf coloured by chain, side view.
PDB entry 6ydf coloured by chain, top view.
Source organism: Collariella virescens
Primary publication:
Oligosaccharide Binding and Thermostability of Two Related AA9 Lytic Polysaccharide Monooxygenases.
Tandrup T, Tryfona T, Frandsen KEH, Johansen KS, Dupree P, Lo Leggio L
Biochemistry 59 3347-3358 (2020)
PMID: 32818374

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103960 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AA9 family lytic polysaccharide monooxygenase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 27.79 KDa
Source organism: Collariella virescens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A223GEC9 (Residues: 23-274; Coverage: 100%)
Gene name: AA9A
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

2 bound ligands:
Ligand CU 2 x CU
Ligand SO4 3 x SO4
1 modified residue:
Ligand HIC 2 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P21
Unit cell:
a: 51.12Å b: 39.39Å c: 119.93Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.2 0.197 0.249
Expression system: Aspergillus oryzae

Quick links

Citations

3 review citations

Lytic polysaccharide monooxygenases and other histidine-brace copper proteins: structure, oxygen activation and biotechnological applications.
Ipsen et al. (2021)
2 more