6ycc

X-ray diffraction
1.3Å resolution

Structure the ananain protease from Ananas comosus covalently bound to the E64 inhibitor

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins with broad specificity for peptide bonds. Best reported small molecule substrate Bz-Phe-Val-Arg-|-NHMec, but broader specificity than fruit bromelain.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ananain Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.45 KDa
Source organism: Ananas comosus
UniProt:
  • Canonical: P80884 (Residues: 123-338; Coverage: 67%)
Gene name: AN1
Sequence domains: Papain family cysteine protease

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 34.324Å b: 59.486Å c: 119.061Å
α: 90° β: 92.86° γ: 90°
R-values:
R R work R free
0.145 0.144 0.168