› 6yca

X-ray diffraction
2.9Å resolution

Crystal structure of Eis1 from Mycobacterium abscessus

Released:
DOI: 10.2210/pdb6yca/pdb
PDB entry 6yca coloured by chain, front view.
PDB entry 6yca coloured by chain, side view.
PDB entry 6yca coloured by chain, top view.
Source organism: Mycobacteroides abscessus
Primary publication:
Structural analysis of the N-acetyltransferase Eis1 from Mycobacterium abscessus reveals the molecular determinants of its incapacity to modify aminoglycosides.
Ung KL, Kremer L, Blaise M
Proteins 89 94-106 (2021)
PMID: 32860271

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GNAT family N-acetyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 415 amino acids
Theoretical weight: 44.9 KDa
Source organism: Mycobacteroides abscessus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A418LDP6 (Residues: 2-415; Coverage: 100%)
Gene name: D2E76_00625
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACO 6 x ACO
Ligand SO4 23 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 118.39Å b: 150.69Å c: 166.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Virulence-Associated Secretion in Mycobacterium abscessus.
Bar-Oz et al. (2022)