Protein Data Bank in Europe

Hydrolysis of proteins in presence of ATP.

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

ATP + kanamycin = ADP + kanamycin 3'-phosphate

4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 

A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol

ATP + D-xylulose = ADP + D-xylulose 5-phosphate

Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

Chloride + O(2) = chlorite

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine

ATP = 3',5'-cyclic AMP + diphosphate

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.

Release of N-terminal proline from a peptide.

Linoleate + O(2) = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate

8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate

Glycerol = 3-hydroxypropanal + H(2)O

Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

Cleavage of peptide bonds with very broad specificity.

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

Chorismate = prephenate

L-asparagine + H(2)O = L-aspartate + NH(3)

Urea + H(2)O = CO(2) + 2 NH(3)

L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate

L-serine + L-homocysteine = L-cystathionine + H(2)O

Triacylglycerol + H(2)O = diacylglycerol + a carboxylate

A carboxylic ester + H(2)O = an alcohol + a carboxylate

4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]

(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Myristoyl-CoA + acetyl-CoA = 3-oxopalmitoyl-CoA + CoA

(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

Nitrous oxide + 2 ferricytochrome c + H(2)O = 2 nitric oxide + 2 ferrocytochrome c + 2 H(+)

Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH

ATP + L-fuculose = ADP + L-fuculose 1-phosphate

Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)

An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

ATP + H(2)O = ADP + phosphate

(R)-10-hydroxystearate = oleate + H(2)O

NTP + H(2)O = NDP + phosphate

The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.

L-fucopyranose = L-fuculose

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA

ATP + pyruvate = ADP + phosphoenolpyruvate

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate

ATP + (d)CMP = ADP + (d)CDP

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

Isocitrate = succinate + glyoxylate

A beta-lactam + H(2)O = a substituted beta-amino acid

3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate

2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH

GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O

RX + glutathione = HX + R-S-glutathione

(1a) S-adenosyl-L-methionine + L-histidine = S-adenosyl-L-homocysteine + N(alpha)-methyl-L-histidine

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

D-glyceraldehyde 3-phosphate = glycerone phosphate

D-ribose 5-phosphate = D-ribulose 5-phosphate

D-mannose = D-fructose

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanoyl-CoA + propanoyl-CoA = CoA + 3-alpha,7-alpha,12-alpha-trihydroxy-24-oxo-5-beta-cholestanoyl-CoA

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

ATP + thiamine phosphate = ADP + thiamine diphosphate

L-carnitine + NAD(P)H + O(2) = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)(+) + H(2)O

Cutin + H(2)O = cutin monomers

Propane-1,2-diol = propanal + H(2)O

2 H(2)O(2) = O(2) + 2 H(2)O

Peptidylproline (omega=180) = peptidylproline (omega=0)

An aldehyde + H(2)O + 2 oxidized ferredoxin = a carboxylate + 2 H(+) + 2 reduced ferredoxin

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans

[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units

(S)-malate + NAD(+) = oxaloacetate + NADH

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

4-aminobutanal + NAD(+) + H(2)O = 4-aminobutanoate + NADH

(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate

(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

Glycerone phosphate = methylglyoxal + phosphate

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate

(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH

Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate

Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH

6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH

Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH

(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH

L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)

H(2) + NAD(+) = H(+) + NADH

Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

(S)-lactate + NAD(+) = pyruvate + NADH

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

Methyl-CoM + CoB = CoM-S-S-CoB + methane

Formamide + H(2)O = formate + NH(3)

Alpha-D-glucosamine 6-phosphate + H(2)O = D-fructose 6-phosphate + NH(3)

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)

Succinate + a quinone = fumarate + a quinol

H(2) + A = AH(2)

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O

Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine

n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

1-haloalkane + H(2)O = a primary alcohol + halide

10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH

4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O

ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp

UDP-3-O-((3R)-3-hydroxyacyl)-N-acetyl-alpha-D-glucosamine + H(2)O = UDP-3-O-((3R)-3-hydroxyacyl)-alpha-D-glucosamine + acetate