› 6ybo

X-ray diffraction
1.06Å resolution

RT structure of Glucose Isomerase obtained at 1.06 A resolution from crystal grown in a Kapton microchip.

Released:
DOI: 10.2210/pdb6ybo/pdb
PDB 6ybo coloured by chain and viewed from the front.
PDB 6ybo coloured by chain and viewed from the side.
PDB 6ybo coloured by chain and viewed from the top.
Source organism: Streptomyces rubiginosus
Primary publication:
Attaining atomic resolution from in situ data collection at room temperature using counter-diffusion-based low-cost microchips.
Gavira JA, Rodriguez-Ruiz I, Martinez-Rodriguez S, Basu S, Teychené S, McCarthy AA, Mueller-Dieckman C
Acta Crystallogr D Struct Biol 76 751-758 (2020)
PMID: 32744257

Function and Biology Details

Reaction catalysed: 
D-xylopyranose = D-xylulose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.28 KDa
Source organism: Streptomyces rubiginosus
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: I222
Unit cell:
a: 93.94Å b: 99.37Å c: 103.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.123 0.131

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