› 6y71

X-ray diffraction
1.64Å resolution

Pseudomonas stutzeri nitrous oxide reductase mutant, H130A

Released:
DOI: 10.2210/pdb6y71/pdb
PDB entry 6y71 coloured by chain, front view.
PDB entry 6y71 coloured by chain, side view.
PDB entry 6y71 coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
A [3Cu:2S] cluster provides insight into the assembly and function of the CuZ site of nitrous oxide reductase.
Zhang L, Bill E, Kroneck PMH, Einsle O
Chem Sci 12 3239-3244 (2021)
PMID: 34164092

Function and Biology Details

Reaction catalysed: 
Nitrogen + H(2)O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148648 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrous-oxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 71.89 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli
UniProt:
  • Canonical: P19573 (Residues: 1-638; Coverage: 100%)
Gene name: nosZ
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand FMT 3 x FMT
Ligand B3P 2 x B3P
Ligand NA 2 x NA
Ligand CA 2 x CA
Ligand CL 2 x CL
Ligand CUA 2 x CUA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 69.156Å b: 76.213Å c: 109.016Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.169 0.169 0.211
Expression system: Escherichia coli

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