› 6y34

X-ray diffraction
1.31Å resolution

Streptavidin wildtype with a biotC5-1 cofactor - an artificial iron hydroxylase

Released:
DOI: 10.2210/pdb6y34/pdb
PDB entry 6y34 coloured by chain, front view.
PDB entry 6y34 coloured by chain, side view.
PDB entry 6y34 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Enantioselective Hydroxylation of Benzylic C(sp3)-H Bonds by an Artificial Iron Hydroxylase Based on the Biotin-Streptavidin Technology.
Serrano-Plana J, Rumo C, Rebelein JG, Peterson RL, Barnet M, Ward TR
J Am Chem Soc 142 10617-10623 (2020)
PMID: 32450689

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: AAA
Molecule details ›
Chain: AAA
Length: 159 amino acids
Theoretical weight: 16.57 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P22629 (Residues: 39-183; Coverage: 91%)
Sequence domains: Avidin family

Ligands and Environments

2 bound ligands:
Ligand O7Q 1 x O7Q
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 57.941Å b: 57.941Å c: 183.822Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.198
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
2 more