› 6y25

X-ray diffraction
1.95Å resolution

Streptavidin mutant S112R,K121E with a biotC4-1 cofactor - an artificial iron hydroxylase

Released:
DOI: 10.2210/pdb6y25/pdb
PDB entry 6y25 coloured by chain, front view.
PDB entry 6y25 coloured by chain, side view.
PDB entry 6y25 coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Enantioselective Hydroxylation of Benzylic C(sp3)-H Bonds by an Artificial Iron Hydroxylase Based on the Biotin-Streptavidin Technology.
Serrano-Plana J, Rumo C, Rebelein JG, Peterson RL, Barnet M, Ward TR
J Am Chem Soc 142 10617-10623 (2020)
PMID: 32450689

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: AAA
Molecule details ›
Chain: AAA
Length: 159 amino acids
Theoretical weight: 16.64 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P22629 (Residues: 39-183; Coverage: 91%)
Sequence domains: Avidin family

Ligands and Environments

1 bound ligand:
Ligand O6T 1 x O6T
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4122
Unit cell:
a: 57.146Å b: 57.146Å c: 182.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.204
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

C-H Bond Cleavage by Bioinspired Nonheme Metal Complexes.
Lee et al. (2021)
1 more

2 mentions without citation

Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.
Van Stappen et al. (2022)
1 more