6xzp

Electron Microscopy
3.3Å resolution

Influenza C virus polymerase in complex with chicken ANP32A - Subclass 4

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Polymerase acidic protein Chains: AP1, DP1
Molecule details ›
Chains: AP1, DP1
Length: 709 amino acids
Theoretical weight: 82.03 KDa
Source organism: Influenza C virus (C/Johannesburg/1/66)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9IMP5 (Residues: 1-709; Coverage: 100%)
Gene names: P3, PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chains: BP1, EP1
Molecule details ›
Chains: BP1, EP1
Length: 754 amino acids
Theoretical weight: 86.15 KDa
Source organism: Influenza C virus (C/Johannesburg/1/66)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9IMP4 (Residues: 1-754; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chains: CP1, FP1
Molecule details ›
Chains: CP1, FP1
Length: 920 amino acids
Theoretical weight: 104.27 KDa
Source organism: Influenza C virus (C/Johannesburg/1/66)
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9IMP3 (Residues: 1-774; Coverage: 100%)
Gene name: PB2
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB2
LRRcap domain-containing protein Chain: GP1
Molecule details ›
Chain: GP1
Length: 295 amino acids
Theoretical weight: 33.92 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A1D5P3M1 (Residues: 1-281; Coverage: 100%)
Gene name: ANP32A
Sequence domains: Leucine-rich repeat
RNA (5'-R(*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*GP*CP*CP*UP*UP*UP*U)-3') Chain: IN1
Molecule details ›
Chain: IN1
Length: 47 nucleotides
Theoretical weight: 14.9 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-10664
Expression system: Spodoptera frugiperda