› 6xxq

X-ray diffraction
3Å resolution

Crystal structure of spectinomycin adenyltransferase AAD(9) from Enterococcus faecialis with ATP and spectinomycin

Released:
DOI: 10.2210/pdb6xxq/pdb
PDB entry 6xxq coloured by chain, front view.
PDB entry 6xxq coloured by chain, side view.
PDB entry 6xxq coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural Recognition of Spectinomycin by Resistance Enzyme ANT(9) from Enterococcus faecalis.
Kanchugal P S, Selmer M
Antimicrob Agents Chemother 64 (2020)
PMID: 32253216

Function and Biology Details

Reaction catalysed: 
ATP + streptomycin = diphosphate + 3''-adenylylstreptomycin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectinomycin 9-adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.87 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07448 (Residues: 1-255; Coverage: 100%)
Gene names: aad9, spc
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand MG 3 x MG
Ligand ATP 2 x ATP
Ligand SCM 2 x SCM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 133.532Å b: 69.312Å c: 94.517Å
α: 90° β: 135° γ: 90°
R-values:
R R work R free
0.241 0.24 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Synthesis, antibacterial action, and ribosome inhibition of deoxyspectinomycins.
Dharuman et al. (2021)
3 more

1 mention without citation

Structural Recognition of Spectinomycin by Resistance Enzyme ANT(9) from Enterococcus faecalis.
Kanchugal P et al. (2020)