6xxm

X-ray diffraction
1.67Å resolution

Crystal Structure of Human Deoxyhypusine Synthase in complex with putrescine

Released:

Function and Biology Details

Reaction catalysed:
(1a) spermidine + NAD(+) = dehydrospermidine + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155938 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Deoxyhypusine synthase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49366 (Residues: 1-369; Coverage: 100%)
Gene names: DHPS, DS
Sequence domains: Deoxyhypusine synthase
Deoxyhypusine synthase Chain: B
Molecule details ›
Chain: B
Length: 369 amino acids
Theoretical weight: 41.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49366 (Residues: 1-369; Coverage: 100%)
Gene names: DHPS, DS
Sequence domains: Deoxyhypusine synthase

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3221
Unit cell:
a: 104.734Å b: 104.734Å c: 160.672Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.161 0.16 0.175
Expression system: Escherichia coli BL21(DE3)