› 6xtl

X-ray diffraction
1.8Å resolution

Crystal structure reveals non-coordinative binding of O2 to the copper center of the formylglycine-generating enzyme - FGE:Ag:S complex

Released:
DOI: 10.2210/pdb6xtl/pdb
PDB entry 6xtl coloured by chain, front view.
PDB entry 6xtl coloured by chain, side view.
PDB entry 6xtl coloured by chain, top view.
Source organism: Thermomonospora curvata DSM 43183
Primary publication:
Non-Coordinative Binding of O2 at the Active Center of a Copper-Dependent Enzyme
Leisinger F, Miarzlou DA, Seebeck FP
Angew. Chem. Int. Ed. Engl. (2020)

Function and Biology Details

Reaction catalysed: 
A [sulfatase]-L-cysteine + O(2) + 2 a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Formylglycine-generating enzyme Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 33.34 KDa
Source organism: Thermomonospora curvata DSM 43183
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D1A7C3 (Residues: 1-302; Coverage: 100%)
Gene name: Tcur_4811
Sequence domains: Sulfatase-modifying factor enzyme 1
Sulfatase N-terminal domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 16 amino acids
Theoretical weight: 1.53 KDa
Source organism: Thermomonospora curvata DSM 43183
Expression system: Escherichia coli
UniProt:
  • Canonical: D1ADF2 (Residues: 76-91; Coverage: 4%)
Gene name: Tcur_0054

Ligands and Environments

5 bound ligands:
Ligand AG 1 x AG
Ligand CA 2 x CA
Ligand EDO 2 x EDO
Ligand CL 1 x CL
Ligand SOA 1 x SOA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 58.283Å b: 72.009Å c: 76.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

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