› 6xkj

Electron Microscopy
3.54Å resolution

Cryo-EM structure of CARD8-CARD filament

Released:
DOI: 10.2210/pdb6xkj/pdb
PDB entry 6xkj coloured by chain, front view.
PDB entry 6xkj coloured by chain, side view.
PDB entry 6xkj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of filament formation in UPA-promoted CARD8 and NLRP1 inflammasomes.
Robert Hollingsworth L, David L, Li Y, Griswold AR, Ruan J, Sharif H, Fontana P, Orth-He EL, Fu TM, Bachovchin DA, Wu H
Nat Commun 12 189 (2021)
PMID: 33420033
Related structures: EMD-22219

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase recruitment domain-containing protein 8, C-terminus Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 87 amino acids
Theoretical weight: 10.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y2G2 (Residues: 451-537; Coverage: 16%)
Gene names: CARD8, DACAR, KIAA0955, NDPP1
Sequence domains: Caspase recruitment domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.54Å
Relevant EMDB volumes: EMD-22219
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structural Mechanisms of NLRP3 Inflammasome Assembly and Activation.
Fu et al. (2023)
13 more