X-ray diffraction
2.05Å resolution

KPC-2 N170A mutant bound to hydrolyzed imipenem at 2.05 A


Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Carbapenem-hydrolyzing beta-lactamase KPC Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 31.1 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9F663 (Residues: 1-293; Coverage: 100%)
Gene names: bla, kpc, kpc1
Sequence domains: Beta-lactamase enzyme family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 56.599Å b: 59.36Å c: 77.62Å
α: 90° β: 90° γ: 90°
R R work R free
0.188 0.185 0.238
Expression system: Escherichia coli BL21(DE3)