X-ray diffraction
2.7Å resolution

Class C beta-lactamase from Escherichia coli in complex with Tazobactam

Source organism: Escherichia coli
Entry authors: Chang C, Maltseva N, Endres M, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 39.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P00811 (Residues: 19-377; Coverage: 100%)
Gene names: JW4111, ampA, ampC, b4150
Sequence domains: Beta-lactamase

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 46.915Å b: 170.299Å c: 89.947Å
α: 90° β: 89.98° γ: 90°
R R work R free
0.191 0.188 0.249
Expression system: Escherichia coli BL21(DE3)