› 6xdr

Electron Microscopy
4.7Å resolution

Escherichia coli transcription-translation complex B (TTC-B) containing an 27 nt long mRNA spacer, NusG, and fMet-tRNAs at E-site and P-site

Released:
DOI: 10.2210/pdb6xdr/pdb
PDB 6xdr coloured by chain and viewed from the front.
PDB 6xdr coloured by chain and viewed from the side.
PDB 6xdr coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural basis of transcription-translation coupling.
Wang C, Molodtsov V, Firlar E, Kaelber JT, Blaha G, Su M, Ebright RH
Science (2020)
PMID: 32820061
Related structures: EMD-22142

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 67-mer (preferred)
Entry contents:
58 distinct polypeptide molecules
2 distinct DNA molecules
5 distinct RNA molecules
Macromolecules (65 distinct):
50S ribosomal protein L21 Chain: 0
Molecule details ›
Chain: 0
Length: 103 amino acids
Theoretical weight: 11.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZEN7 (Residues: 1-103; Coverage: 100%)
Gene names: HMPREF9534_02716, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
50S ribosomal protein L22 Chain: 1
Molecule details ›
Chain: 1
Length: 110 amino acids
Theoretical weight: 12.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G0 (Residues: 1-110; Coverage: 100%)
Gene names: HMPREF9534_00853, rplV
Sequence domains: Ribosomal protein L22p/L17e
50S ribosomal protein L23 Chain: 2
Molecule details ›
Chain: 2
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7X2Z9 (Residues: 1-100; Coverage: 100%)
Gene names: HMPREF9552_00977, rplW
Sequence domains: Ribosomal protein L23
50S ribosomal protein L24 Chain: 3
Molecule details ›
Chain: 3
Length: 104 amino acids
Theoretical weight: 11.34 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G6 (Residues: 1-104; Coverage: 100%)
Gene names: HMPREF9534_00859, rplX
Sequence domains:
50S ribosomal protein L25 Chain: 4
Molecule details ›
Chain: 4
Length: 94 amino acids
Theoretical weight: 10.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7XH79 (Residues: 1-94; Coverage: 100%)
Gene names: HMPREF9536_00351, rplY
Sequence domains: Ribosomal L25p family
50S ribosomal protein L10 Chain: 9
Molecule details ›
Chain: 9
Length: 165 amino acids
Theoretical weight: 17.74 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZA73 (Residues: 1-165; Coverage: 100%)
Gene names: HMPREF9534_01121, rplJ
Sequence domains: Ribosomal protein L10
DNA-directed RNA polymerase subunit beta Chain: AA
Molecule details ›
Chain: AA
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V4 (Residues: 1-1342; Coverage: 100%)
Gene names: ECs4910, Z5560, rpoB
Sequence domains:
Transcription termination/antitermination protein NusG Chain: AB
Molecule details ›
Chain: AB
Length: 181 amino acids
Theoretical weight: 20.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: V8K3D7 (Residues: 1-181; Coverage: 100%)
Gene names: V415_18420, nusG
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chains: AC, AD
Molecule details ›
Chains: AC, AD
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A073G207 (Residues: 1-329; Coverage: 100%)
Gene names: AD31_3960, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: AE
Molecule details ›
Chain: AE
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A4S1NBU2 (Residues: 1-1407; Coverage: 100%)
Gene names: BvCmsKKP036_03580, G5696_22040, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: AF
Molecule details ›
Chain: AF
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A802 (Residues: 1-91; Coverage: 100%)
Gene names: ECs4524, Z5075, rpoZ
Sequence domains: RNA polymerase Rpb6
30S ribosomal protein S18 Chain: C
Molecule details ›
Chain: C
Length: 75 amino acids
Theoretical weight: 9.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0E2KXL3 (Residues: 1-75; Coverage: 100%)
Gene names: G925_04532, rpsR
Sequence domains: Ribosomal protein S18
30S ribosomal protein S20 Chain: E
Molecule details ›
Chain: E
Length: 87 amino acids
Theoretical weight: 9.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZAS2 (Residues: 1-87; Coverage: 100%)
Gene names: HMPREF9534_01320, rpsT
Sequence domains: Ribosomal protein S20
30S ribosomal protein S21 Chain: F
Molecule details ›
Chain: F
Length: 71 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0E2L2J1 (Residues: 1-71; Coverage: 100%)
Gene names: G925_03304, rpsU
Sequence domains: Ribosomal protein S21
30S ribosomal protein S2 Chain: G
Molecule details ›
Chain: G
Length: 241 amino acids
Theoretical weight: 26.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZK99 (Residues: 1-241; Coverage: 100%)
Gene names: HMPREF9534_04716, rpsB
Sequence domains: Ribosomal protein S2
30S ribosomal protein S1 Chain: H
Molecule details ›
Chain: H
Length: 557 amino acids
Theoretical weight: 61.24 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D6HUW2 (Residues: 1-557; Coverage: 100%)
Gene name: ECCG_01333
Sequence domains: S1 RNA binding domain
30S ribosomal protein S3 Chain: I
Molecule details ›
Chain: I
Length: 233 amino acids
Theoretical weight: 26.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MCS9 (Residues: 1-233; Coverage: 100%)
Gene names: ECS88_3701, rpsC
Sequence domains:
30S ribosomal protein S4 Chain: J
Molecule details ›
Chain: J
Length: 206 amino acids
Theoretical weight: 23.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9H7 (Residues: 1-206; Coverage: 100%)
Gene names: HMPREF9534_00870, rpsD
Sequence domains:
30S ribosomal protein S5 Chain: K
Molecule details ›
Chain: K
Length: 167 amino acids
Theoretical weight: 17.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9H2 (Residues: 1-167; Coverage: 100%)
Gene names: HMPREF9534_00865, rpsE
Sequence domains:
30S ribosomal protein S6 Chain: L
Molecule details ›
Chain: L
Length: 135 amino acids
Theoretical weight: 15.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-135; Coverage: 100%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
30S ribosomal protein S7 Chain: M
Molecule details ›
Chain: M
Length: 179 amino acids
Theoretical weight: 20.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
30S ribosomal protein S8 Chain: N
Molecule details ›
Chain: N
Length: 130 amino acids
Theoretical weight: 14.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G9 (Residues: 1-130; Coverage: 100%)
Gene names: HMPREF9534_00862, rpsH
Sequence domains: Ribosomal protein S8
30S ribosomal protein S9 Chain: O
Molecule details ›
Chain: O
Length: 130 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZES9 (Residues: 1-130; Coverage: 100%)
Gene names: HMPREF9534_02759, rpsI
Sequence domains: Ribosomal protein S9/S16
30S ribosomal protein S10 Chain: P
Molecule details ›
Chain: P
Length: 103 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9F4 (Residues: 1-103; Coverage: 100%)
Gene names: HMPREF9534_00847, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
30S ribosomal protein S11 Chain: Q
Molecule details ›
Chain: Q
Length: 129 amino acids
Theoretical weight: 13.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MCR3 (Residues: 1-129; Coverage: 100%)
Gene names: ECS88_3685, rpsK
Sequence domains: Ribosomal protein S11
30S ribosomal protein S12 Chain: R
Molecule details ›
Chain: R
Length: 124 amino acids
Theoretical weight: 13.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZAN0 (Residues: 1-124; Coverage: 100%)
Gene names: HMPREF9534_01278, rpsL
Sequence domains: Ribosomal protein S12/S23
30S ribosomal protein S14 Chain: S
Molecule details ›
Chain: S
Length: 101 amino acids
Theoretical weight: 11.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G8 (Residues: 1-101; Coverage: 100%)
Gene names: HMPREF9534_00861, rpsN
Sequence domains: Ribosomal protein S14p/S29e
30S ribosomal protein S15 Chain: T
Molecule details ›
Chain: T
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZEL4 (Residues: 1-89; Coverage: 100%)
Gene names: HMPREF9534_02691, rpsO
Sequence domains: Ribosomal protein S15
30S ribosomal protein S16 Chain: U
Molecule details ›
Chain: U
Length: 82 amino acids
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MIU7 (Residues: 1-82; Coverage: 100%)
Gene names: ECS88_2795, rpsP
Sequence domains: Ribosomal protein S16
30S ribosomal protein S17 Chain: V
Molecule details ›
Chain: V
Length: 84 amino acids
Theoretical weight: 9.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G4 (Residues: 1-84; Coverage: 100%)
Gene names: HMPREF9534_00857, rpsQ
Sequence domains: Ribosomal protein S17
30S ribosomal protein S19 Chain: W
Molecule details ›
Chain: W
Length: 92 amino acids
Theoretical weight: 10.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9F9 (Residues: 1-92; Coverage: 100%)
Gene names: HMPREF9534_00852, rpsS
Sequence domains: Ribosomal protein S19
30S ribosomal protein S13 Chain: X
Molecule details ›
Chain: X
Length: 118 amino acids
Theoretical weight: 13.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: H4UQ02 (Residues: 1-118; Coverage: 100%)
Gene names: ECDEC6A_4055, rpsM
Sequence domains: Ribosomal protein S13/S18
50S ribosomal protein L11 Chain: Y
Molecule details ›
Chain: Y
Length: 142 amino acids
Theoretical weight: 14.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZA71 (Residues: 1-142; Coverage: 100%)
Gene names: HMPREF9534_01119, rplK
Sequence domains:
50S ribosomal protein L7/L12 Chain: Z
Molecule details ›
Chain: Z
Length: 121 amino acids
Theoretical weight: 12.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: H4KQ39 (Residues: 1-121; Coverage: 100%)
Gene names: ECDEC2C_4837, rplL
Sequence domains:
50S ribosomal protein L27 Chain: b
Molecule details ›
Chain: b
Length: 85 amino acids
Theoretical weight: 9.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 1-85; Coverage: 100%)
Gene names: JW3152, b3185, rpmA
Sequence domains: Ribosomal L27 protein
50S ribosomal protein L28 Chain: c
Molecule details ›
Chain: c
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: S0YB49 (Residues: 1-78; Coverage: 100%)
Gene names: WG7_04421, rpmB
Sequence domains: Ribosomal L28 family
50S ribosomal protein L29 Chain: e
Molecule details ›
Chain: e
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G3 (Residues: 1-63; Coverage: 100%)
Gene names: HMPREF9534_00856, rpmC
Sequence domains: Ribosomal L29 protein
50S ribosomal protein L30 Chain: f
Molecule details ›
Chain: f
Length: 59 amino acids
Theoretical weight: 6.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9H3 (Residues: 1-59; Coverage: 100%)
Gene names: HMPREF9534_00866, rpmD
Sequence domains: Ribosomal protein L30p/L7e
50S ribosomal protein L31 Chain: g
Molecule details ›
Chain: g
Length: 70 amino acids
Theoretical weight: 7.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZFP6 (Residues: 1-70; Coverage: 100%)
Gene names: HMPREF9534_03082, rpmE
Sequence domains: Ribosomal protein L31
50S ribosomal protein L2 Chain: h
Molecule details ›
Chain: h
Length: 273 amino acids
Theoretical weight: 29.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60422 (Residues: 1-273; Coverage: 100%)
Gene names: JW3279, b3317, rplB
Sequence domains:
50S ribosomal protein L32 Chain: i
Molecule details ›
Chain: i
Length: 57 amino acids
Theoretical weight: 6.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZGG2 (Residues: 1-57; Coverage: 100%)
Gene names: HMPREF9534_03351, rpmF
Sequence domains: Ribosomal L32p protein family
50S ribosomal protein L3 Chain: j
Molecule details ›
Chain: j
Length: 209 amino acids
Theoretical weight: 22.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P60438 (Residues: 1-209; Coverage: 100%)
Gene names: JW3282, b3320, rplC
Sequence domains: Ribosomal protein L3
50S ribosomal protein L33 Chain: k
Molecule details ›
Chain: k
Length: 55 amino acids
Theoretical weight: 6.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9P0 (Residues: 1-55; Coverage: 100%)
Gene names: HMPREF9534_00935, rpmG
Sequence domains: Ribosomal protein L33
50S ribosomal protein L4 Chain: l
Molecule details ›
Chain: l
Length: 201 amino acids
Theoretical weight: 22.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9F6 (Residues: 1-201; Coverage: 100%)
Gene names: HMPREF9534_00849, rplD
Sequence domains: Ribosomal protein L4/L1 family
50S ribosomal protein L34 Chain: m
Molecule details ›
Chain: m
Length: 46 amino acids
Theoretical weight: 5.4 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MGC4 (Residues: 1-46; Coverage: 100%)
Gene names: ECS88_4126, rpmH
Sequence domains: Ribosomal protein L34
50S ribosomal protein L5 Chain: n
Molecule details ›
Chain: n
Length: 179 amino acids
Theoretical weight: 20.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G7 (Residues: 1-179; Coverage: 100%)
Gene names: HMPREF9534_00860, rplE
Sequence domains:
50S ribosomal protein L35 Chain: o
Molecule details ›
Chain: o
Length: 65 amino acids
Theoretical weight: 7.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z814 (Residues: 1-65; Coverage: 100%)
Gene names: HMPREF9534_00345, rpmI
Sequence domains: Ribosomal protein L35
50S ribosomal protein L6 Chain: p
Molecule details ›
Chain: p
Length: 177 amino acids
Theoretical weight: 18.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9H0 (Residues: 1-177; Coverage: 100%)
Gene names: HMPREF9534_00863, rplF
Sequence domains: Ribosomal protein L6
50S ribosomal protein L36 Chain: q
Molecule details ›
Chain: q
Length: 38 amino acids
Theoretical weight: 4.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0E2L017 (Residues: 1-38; Coverage: 100%)
Gene names: G925_03522, rpmJ
Sequence domains: Ribosomal protein L36
50S ribosomal protein L9 Chain: r
Molecule details ›
Chain: r
Length: 149 amino acids
Theoretical weight: 15.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZI15 (Residues: 1-149; Coverage: 100%)
Gene names: HMPREF9534_03905, rplI
Sequence domains:
50S ribosomal protein L13 Chain: s
Molecule details ›
Chain: s
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZET0 (Residues: 1-142; Coverage: 100%)
Gene names: HMPREF9534_02760, rplM
Sequence domains: Ribosomal protein L13
50S ribosomal protein L14 Chain: t
Molecule details ›
Chain: t
Length: 123 amino acids
Theoretical weight: 13.57 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9G5 (Residues: 1-123; Coverage: 100%)
Gene names: HMPREF9534_00858, rplN
Sequence domains: Ribosomal protein L14p/L23e
50S ribosomal protein L15 Chain: u
Molecule details ›
Chain: u
Length: 144 amino acids
Theoretical weight: 15.01 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A037Y8L6 (Residues: 1-144; Coverage: 100%)
Gene names: A8C65_19395, A9R57_24660, ACU57_21045, AM464_09135, AUQ13_22145, BANRA_01932, BANRA_03684, BHS87_18545, BJJ90_02210, BMA87_18510, BON75_18850, BON98_13310, BUE81_25610, BW690_10710, BZL31_22885, BvCms2454_01416, BvCmsHHP001_00334, BvCmsHHP019_04131, BvCmsHHP056_02703, BvCmsKSP026_02554, BvCmsSINP011_02480, C5F72_02595, C5F73_02860, C5N07_23575, C9E25_20065, CA593_09735, CG692_20270, CI693_13520, CI694_01990, D0X26_24495, D2185_22985, D3821_17755, D3O91_20305, D3Y67_24390, D9C99_17930, D9D20_21995, D9D44_22490, D9G69_20945, D9H68_17710, D9J52_19090, D9Z28_15390, DAH37_14685, DJ503_19770, DNC98_19095, DT034_22410, DTL43_21000, E2119_19640, E2134_21880, E2135_06185, E4K53_21820, E4K55_21420, E4K60_18985, E4K61_20025, E5P28_20875, EA213_19860, EAI42_04990, EAI52_16365, EC1094V2_354, EC3234A_55c00230, ECTO6_00449, EEP23_17135, EG808_17075, EI021_21030, EI032_18995, EI041_20035, EIZ93_12470, EL75_0396, EL79_0415, EL80_0407, EPT01_19765, ERS150873_04051, ETECE1441_00448, ETECE1649_00473, ETECE925_00488, EXX71_21335, EYD11_02080, EYV18_18400, F1E19_19655, FRV13_20015, FV293_22465, FWK02_06270, G5632_17770, G5688_20025, G9P49_02115, G9P50_02025, GHD50_22055, GKF39_20550, GKF74_18130, GKF86_20070, GKF89_18470, GP954_00945, GP979_02005, GQE33_22230, GQE34_14255, GQE64_20420, GQM17_19915, GRW02_22070, GRW05_09060, GRW57_03540, GRW80_20585, GRW81_21150, H4P50_02035, H4P51_02025, HH707_004392, HJV81_003481, HLT96_16065, HLU13_19455, HMQ05_22670, HNN86_20620, HNV44_20240, HPE39_23335, HV005_02090, HVV53_13950, HVX22_02040, HVX24_02180, HVY77_02175, HVY79_21110, HVY93_02055, HVZ24_02045, HX136_02195, NCTC10963_00456, NCTC11022_03475, NCTC11126_02772, NCTC13216_01351, NCTC9045_00567, NCTC9062_02686, NCTC9117_00757, NCTC9706_02738, PGD_00585, RK56_021420, SAMEA3472047_03862, SAMEA3472080_04748, SAMEA3484427_04887, SAMEA3484429_04875, SAMEA3752559_00346, SAMEA3753300_02227, WP2S18E08_04080, WP7S17E04_04090, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
50S ribosomal protein L16 Chain: v
Molecule details ›
Chain: v
Length: 136 amino acids
Theoretical weight: 15.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADY7 (Residues: 1-136; Coverage: 100%)
Gene names: JW3275, b3313, rplP
Sequence domains: Ribosomal protein L16p/L10e
50S ribosomal protein L17 Chain: w
Molecule details ›
Chain: w
Length: 127 amino acids
Theoretical weight: 14.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D6I1Y5 (Residues: 1-127; Coverage: 100%)
Gene names: ECCG_03701, rplQ
Sequence domains: Ribosomal protein L17
50S ribosomal protein L18 Chain: x
Molecule details ›
Chain: x
Length: 117 amino acids
Theoretical weight: 12.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7Z9H1 (Residues: 1-117; Coverage: 100%)
Gene names: HMPREF9534_00864, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
50S ribosomal protein L19 Chain: y
Molecule details ›
Chain: y
Length: 115 amino acids
Theoretical weight: 13.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D7ZKU3 (Residues: 1-115; Coverage: 100%)
Gene names: HMPREF9534_04913, rplS
Sequence domains: Ribosomal protein L19
50S ribosomal protein L20 Chain: z
Molecule details ›
Chain: z
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B7MAS6 (Residues: 1-118; Coverage: 100%)
Gene names: ECS88_1766, rplT
Sequence domains: Ribosomal protein L20
NT DNA Chain: 5
Molecule details ›
Chain: 5
Length: 36 nucleotides
Theoretical weight: 11.04 KDa
T DNA Chain: 6
Molecule details ›
Chain: 6
Length: 36 nucleotides
Theoretical weight: 10.96 KDa
mRNA with 27 nt long spacer Chain: 7
Molecule details ›
Chain: 7
Length: 44 nucleotides
Theoretical weight: 13.83 KDa
E-site and P-site tRNA (fMet) Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 nucleotides
Theoretical weight: 24.5 KDa
16S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
23S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 2904 nucleotides
Theoretical weight: 941.64 KDa
5S ribosomal RNA Chain: d
Molecule details ›
Chain: d
Length: 120 nucleotides
Theoretical weight: 38.79 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.7Å
Relevant EMDB volumes: EMD-22142
Expression system: Escherichia coli

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Citations

3 review citations

Coupled Transcription-Translation in Prokaryotes: An Old Couple With New Surprises.
Irastortza-Olaziregi et al. (2020)
2 more