› 6xc3

X-ray diffraction
2.7Å resolution

Crystal structure of SARS-CoV-2 receptor binding domain in complex with antibodies CC12.1 and CR3022

Released:
DOI: 10.2210/pdb6xc3/pdb
PDB 6xc3 coloured by chain and viewed from the front.
PDB 6xc3 coloured by chain and viewed from the side.
PDB 6xc3 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural basis of a shared antibody response to SARS-CoV-2.
Yuan M, Liu H, Wu NC, Lee CD, Zhu X, Zhao F, Huang D, Yu W, Hua Y, Tien H, Rogers TF, Landais E, Sok D, Jardine JG, Burton DR, Wilson IA
Science 369 1119-1123 (2020)
PMID: 32661058

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
CR3022 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CR3022 light chain Chain: L
Molecule details ›
Chain: L
Length: 221 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
CC12.1 light chain Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 23.64 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
CC12.1 heavy chain Chain: B
Molecule details ›
Chain: B
Length: 220 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
Spike protein S1 Chain: C
Molecule details ›
Chain: C
Length: 231 amino acids
Theoretical weight: 26.1 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0DTC2 (Residues: 319-541; Coverage: 18%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P41212
Unit cell:
a: 109.772Å b: 109.772Å c: 235.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.225
Expression systems:
  • Homo sapiens
  • Mus musculus
  • Trichoplusia ni

Quick links

Citations

4 review citations

Structural Analysis of Neutralizing Epitopes of the SARS-CoV-2 Spike to Guide Therapy and Vaccine Design Strategies.
Finkelstein et al. (2021)
3 more