› 6xb3

X-ray diffraction
1.9Å resolution

Structure of AcNPV poxin in post-reactive state with Gp[2'-5']Ap[3']

Released:
DOI: 10.2210/pdb6xb3/pdb
PDB entry 6xb3 coloured by chain, front view.
PDB entry 6xb3 coloured by chain, side view.
PDB entry 6xb3 coloured by chain, top view.
Source organism: Autographa californica nucleopolyhedrovirus
Primary publication:
Structures of diverse poxin cGAMP nucleases reveal a widespread role for cGAS-STING evasion in host-pathogen conflict.
Eaglesham JB, McCarty KL, Kranzusch PJ
Elife 9 (2020)
PMID: 33191912

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poxin Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 241 amino acids
Theoretical weight: 27.4 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08358 (Residues: 1-240; Coverage: 100%)
Gene name: P26
Sequence domains: Nucleopolyhedrovirus p26 protein

Ligands and Environments

1 bound ligand:
Ligand 9BG 16 x 9BG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 127.234Å b: 127.192Å c: 127.151Å
α: 124.813° β: 102.39° γ: 102.385°
R-values:
R R work R free
0.186 0.175 0.208
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

The Trinity of cGAS, TLR9, and ALRs Guardians of the Cellular Galaxy Against Host-Derived Self-DNA.
Kumar V. (2020)
7 more

2 mentions without citation

Structures of diverse poxin cGAMP nucleases reveal a widespread role for cGAS-STING evasion in host-pathogen conflict.
Eaglesham et al. (2020)
1 more