› 6x8x

X-ray diffraction
2.5Å resolution

Cu-bound structure of an engineered metal-dependent protein trimer, TriCyt1

Released:
DOI: 10.2210/pdb6x8x/pdb
PDB entry 6x8x coloured by chain, front view.
PDB entry 6x8x coloured by chain, side view.
PDB entry 6x8x coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis A, Gagnon D, Esselborn J, Britt RD, Tezcan FA
Angew Chem Int Ed Engl 59 21940-21944 (2020)
PMID: 32830423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand HEC 1 x HEC
Ligand CU 1 x CU
Ligand CA 5 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P321
Unit cell:
a: 82.172Å b: 82.172Å c: 48.072Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.285 0.342
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
11 other articles

1 mention without citation

Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis et al. (2020)