› 6wz3

X-ray diffraction
1.8Å resolution

Cu-bound structure of the engineered protein trimer, TriCyt3

Released:
DOI: 10.2210/pdb6wz3/pdb
PDB entry 6wz3 coloured by chain, front view.
PDB entry 6wz3 coloured by chain, side view.
PDB entry 6wz3 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis A, Gagnon D, Esselborn J, Britt RD, Tezcan FA
Angew Chem Int Ed Engl 59 21940-21944 (2020)
PMID: 32830423

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142157 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 106 amino acids
Theoretical weight: 11.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

4 bound ligands:
Ligand HEC 3 x HEC
Ligand CL 7 x CL
Ligand CU 1 x CU
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 78.447Å b: 81.205Å c: 56.431Å
α: 90° β: 92.92° γ: 90°
R-values:
R R work R free
0.19 0.188 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protein Assembly by Design.
Zhu et al. (2021)
11 other articles

1 mention without citation

Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis et al. (2020)