› 6wz0

X-ray diffraction
1.7Å resolution

Fe-bound structure of an engineered metal-dependent protein trimer, TriCyt2

Released:
DOI: 10.2210/pdb6wz0/pdb
PDB 6wz0 coloured by chain and viewed from the front.
PDB 6wz0 coloured by chain and viewed from the side.
PDB 6wz0 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites.
Kakkis A, Gagnon D, Esselborn J, Britt RD, Tezcan FA
Angew Chem Int Ed Engl 59 21940-21944 (2020)
PMID: 32830423

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 106 amino acids
Theoretical weight: 11.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand HEC 3 x HEC
Ligand FE2 1 x FE2
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 48.206Å b: 79.26Å c: 49.916Å
α: 90° β: 106.89° γ: 90°
R-values:
R R work R free
0.183 0.182 0.212
Expression system: Escherichia coli

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