› 6wwg

Electron Microscopy
2.9Å resolution

KIF14[391-772] dimer two-heads-bound state - ADP-AlFx in complex with a microtubule

Released:
DOI: 10.2210/pdb6wwg/pdb
PDB entry 6wwg coloured by chain, front view.
PDB entry 6wwg coloured by chain, side view.
PDB entry 6wwg coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14.
Benoit MPMH, Asenjo AB, Paydar M, Dhakal S, Kwok BH, Sosa H
Nat Commun 12 3637 (2021)
PMID: 34131133
Related structures: EMD-21934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-104111 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinesin-like protein KIF14 Chains: K, N
Molecule details ›
Chains: K, N
Length: 390 amino acids
Theoretical weight: 43.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: L0N7N1 (Residues: 391-772; Coverage: 23%)
Gene name: Kif14
Sequence domains: Kinesin motor domain
Tubulin alpha-1B chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin, beta 2B class IIb Chains: B, I
Molecule details ›
Chains: B, I
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AZ37 (Residues: 127-553; Coverage: 77%)
Gene name: TUBB2B
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand AF3 1 x AF3
Ligand ADP 2 x ADP
Ligand MG 3 x MG
Ligand GTP 2 x GTP
Ligand GDP 2 x GDP
Ligand TA1 2 x TA1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-21934
Expression system: Escherichia coli

Quick links

Citations

1 review citation

New insights into the mechanochemical coupling mechanism of kinesin-microtubule complexes from their high-resolution structures.
Benoit et al. (2023)
6 other articles

1 mention without citation

Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14.
Benoit et al. (2021)