› 6ww4

X-ray diffraction
2.25Å resolution

Crystal structure of HERC2 ZZ domain in complex with histone H3 tail

Released:
DOI: 10.2210/pdb6ww4/pdb
PDB entry 6ww4 coloured by chain, front view.
PDB entry 6ww4 coloured by chain, side view.
PDB entry 6ww4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insight into Binding of the ZZ Domain of HERC2 to Histone H3 and SUMO1.
Liu J, Xue Z, Zhang Y, Vann KR, Shi X, Kutateladze TG
Structure 28 1225-1230.e3 (2020)
PMID: 32726574

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HERC2; Histone H3.1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 56 amino acids
Theoretical weight: 6.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68431 (Residues: 2-7; Coverage: 4%)
  • Canonical: O95714 (Residues: 2702-2751; Coverage: 1%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HERC2, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J
Sequence domains: Zinc finger, ZZ type

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 24.933Å b: 76.439Å c: 33.012Å
α: 90° β: 102.52° γ: 90°
R-values:
R R work R free
0.203 0.198 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Structural and biophysical characterization of the nucleosome-binding PZP domain.
Klein et al. (2021)
2 more

4 mentions without citation

Exploring the Roles of HERC2 and the NEDD4L HECT E3 Ubiquitin Ligase Subfamily in p53 Signaling and the DNA Damage Response.
Mathieu et al. (2021)
3 more