› 6wv7

X-ray diffraction
2.48Å resolution

Human VKOR with Chlorophacinone

Released:
DOI: 10.2210/pdb6wv7/pdb
PDB entry 6wv7 coloured by chain, front view.
PDB entry 6wv7 coloured by chain, side view.
PDB entry 6wv7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of antagonizing the vitamin K catalytic cycle for anticoagulation.
Liu S, Li S, Shen G, Sukumar N, Krezel AM, Li W
Science 371 (2021)
PMID: 33154105

Function and Biology Details

Reaction catalysed: 
Phylloquinol + a protein with a disulfide bond = phylloquinone + a protein with reduced L-cysteine residues
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237031 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; Vitamin K epoxide reductase complex subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 44.2 KDa
Source organisms: Expression system: Komagataella pastoris
UniProt:
  • Canonical: P42212 (Residues: 1-145, 146-231; Coverage: 97%)
  • Canonical: Q9BQB6 (Residues: 4-155; Coverage: 93%)
Gene names: GFP, MSTP134, MSTP576, UNQ308/PRO351, VKOR, VKORC1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand UAJ 2 x UAJ
1 modified residue:
Ligand CRO 2 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 42.564Å b: 82.567Å c: 78.782Å
α: 93.39° β: 97.36° γ: 104.21°
R-values:
R R work R free
0.202 0.2 0.245
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

The Effects of Warfarin and Direct Oral Anticoagulants on Systemic Vascular Calcification: A Review.
Elango et al. (2021)
1 more

1 mention without citation

Structural basis of antagonizing the vitamin K catalytic cycle for anticoagulation.
Liu et al. (2021)