› 6wuk

X-ray diffraction
1.6Å resolution

Crystal structure of mouse DXO in complex with CoA

Released:
DOI: 10.2210/pdb6wuk/pdb
PDB 6wuk coloured by chain and viewed from the front.
PDB 6wuk coloured by chain and viewed from the side.
PDB 6wuk coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
DXO/Rai1 enzymes remove 5'-end FAD and dephospho-CoA caps on RNAs.
Doamekpor SK, Grudzien-Nogalska E, Mlynarska-Cieslak A, Kowalska J, Kiledjian M, Tong L
Nucleic Acids Res 48 6136-6148 (2020)
PMID: 32374864

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Decapping and exoribonuclease protein Chain: A
Molecule details ›
Chain: A
Length: 417 amino acids
Theoretical weight: 47.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70348 (Residues: 1-397; Coverage: 100%)
Gene names: Dom3z, Dxo, Ng6
Sequence domains: RAI1 like PD-(D/E)XK nuclease

Ligands and Environments

1 bound ligand:
Ligand COA 1 x COA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21
Unit cell:
a: 49.862Å b: 87.665Å c: 54.023Å
α: 90° β: 112.24° γ: 90°
R-values:
R R work R free
0.186 0.185 0.214
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Eukaryotic mRNA Decapping Activation.
Vidya et al. (2022)
1 more