6wsw

X-ray diffraction
3.12Å resolution

Self-assembly of a 3D DNA crystal lattice (4x5 duplex version) containing the J21 immobile Holliday junction

Released:
Source organism: synthetic construct
Entry authors: Simmons CR, MacCulloch T, Stephanopoulos N, Yan H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct DNA molecules
Macromolecules (4 distinct):
DNA (5'-D(*AP*AP*GP*CP*AP*GP*AP*CP*CP*TP*GP*AP*CP*TP*GP*CP*AP*CP*TP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*GP*TP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*GP*AP*GP*TP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*GP*TP*CP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P32
Unit cell:
a: 69.033Å b: 69.033Å c: 59.371Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.234 0.278
Expression system: Not provided