› 6wsw

X-ray diffraction
3.12Å resolution

Self-assembly of a 3D DNA crystal lattice (4x5 duplex version) containing the J21 immobile Holliday junction

Released:
DOI: 10.2210/pdb6wsw/pdb
PDB 6wsw coloured by chain and viewed from the front.
PDB 6wsw coloured by chain and viewed from the side.
PDB 6wsw coloured by chain and viewed from the top.
Source organism: synthetic construct
Entry authors: Simmons CR, MacCulloch T, Stephanopoulos N, Yan H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct DNA molecules
Macromolecules (4 distinct):
DNA (5'-D(*AP*AP*GP*CP*AP*GP*AP*CP*CP*TP*GP*AP*CP*TP*GP*CP*AP*CP*TP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.4 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*GP*TP*CP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*GP*AP*GP*TP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.75 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*GP*TP*CP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.13 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CAC 2 x CAC
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P32
Unit cell:
a: 69.033Å b: 69.033Å c: 59.371Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.234 0.278
Expression system: Not provided

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