6wsu

X-ray diffraction
2.76Å resolution

Self-assembly of a 3D DNA crystal lattice (4x5 duplex version) containing the J19 immobile Holliday junction

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct DNA molecules
Macromolecules (4 distinct):
DNA (5'-D(*GP*AP*GP*CP*AP*GP*AP*CP*GP*AP*GP*AP*CP*TP*CP*CP*AP*CP*TP*CP*A)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*GP*TP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.5 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*GP*AP*GP*TP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*GP*TP*CP*TP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 7 nucleotides
Theoretical weight: 2.09 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 68.513Å b: 68.513Å c: 60.773Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.211 0.233
Expression system: Not provided