› 6wmb

X-ray diffraction
3.02Å resolution

Crystal structure of a soluble variant of full-length human APOBEC3G (pH 8.0)

Released:
DOI: 10.2210/pdb6wmb/pdb
PDB entry 6wmb coloured by chain, front view.
PDB entry 6wmb coloured by chain, side view.
PDB entry 6wmb coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure of a Soluble APOBEC3G Variant Suggests ssDNA to Bind in a Channel that Extends between the Two Domains.
Maiti A, Myint W, Delviks-Frankenberry KA, Hou S, Kanai T, Balachandran V, Sierra Rodriguez C, Tripathi R, Kurt Yilmaz N, Pathak VK, Schiffer CA, Matsuo H
J Mol Biol 432 6042-6060 (2020)
PMID: 33098858

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-120850 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
APOLIPOPROTEIN B MRNA EDITING ENZYME, CATALYTIC PEPTIDE- LIKE 3G Chain: A
Molecule details ›
Chain: A
Length: 367 amino acids
Theoretical weight: 42.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
DNA (5'-D(P*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 2 nucleotides
Theoretical weight: 533 Da
Source organism: Escherichia coli
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 60.238Å b: 42.081Å c: 194.21Å
α: 90° β: 95.759° γ: 90°
R-values:
R R work R free
0.253 0.247 0.305
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Interactions of APOBEC3s with DNA and RNA.
Maiti et al. (2021)
1 more

2 mentions without citation

Crystal Structure of a Soluble APOBEC3G Variant Suggests ssDNA to Bind in a Channel that Extends between the Two Domains.
Maiti et al. (2020)
1 more