6wks

X-ray diffraction
1.8Å resolution

Structure of SARS-CoV-2 nsp16/nsp10 in complex with RNA cap analogue (m7GpppA) and S-adenosylmethionine

Released:
Entry authors: Gupta YK, Viswanathan T, Arya S, Qi S, Misra A, Chan S-H

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2'-O-methyltransferase Chain: AAA
Molecule details ›
Chain: AAA
Length: 298 amino acids
Theoretical weight: 33.36 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P0DTD1 (Residues: 6799-7096)
Gene names: 1a-1b, rep
Non-structural protein 10 Chain: BBB
Molecule details ›
Chain: BBB
Length: 139 amino acids
Theoretical weight: 14.8 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P0DTD1 (Residues: 4254-4392)
Gene names: 1a-1b, rep

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 168.067Å b: 168.067Å c: 52.304Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.152 0.188
Expression system: Escherichia coli BL21(DE3)